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Abstract Regional innovation system has been changed from innovation ecosystem to innovation entrepreneurial ecosystem to comprehensive innovation ecosystem. How to construct regional comprehensive innovation ecosystem becomes focus point of implementing innovationdriven strategy in cities and districts. The interaction of "Innovation, Entrepreneur, Venture Capital and Maker (IEVM)" is the key factor of comprehensive innovation ecosystem. First, the internal mechanism of interaction of IEVM was analyzed. Second, how to promote interaction of IEVM in Longgang was described. At last, experience of Longgang was summarized: aggregating advanced innovation resources, focusing on makersandentrepreneurs, market leading and interactive development of innovation chain and industry chain. These experience are valuable for those regions who don't have universities or institutions.
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